Surprisingly few labs properly train new scientists in good pipetting technique, but the way you pipette can make a big difference in the reproducibility of your results—both accuracy and precision can suffer. Because we care about good data, we’ve taken the time to systematically test the effects of different factors, such as pipetting technique, on […]
When testing accuracy, precision and trueness of liquid handling instruments, how many data points should you test for each volume? Does pipetting technique impact data reproducibility and accuracy? How often should you calibrate your pipettes? Have you recently thought about your assays and how much variability in liquid handling they can tolerate? This eBook is intended to help users of pipettes and automated liquid handling systems find answers to questions just like these.
This study evaluates a number of techniques that influence the accuracy and precision of the pipetted volume. The pipette operator has the ability to control all of these parameters by using the appropriate pipetting technique, as well as by choosing the appropriate pipette size and type of pipette tips.
Every day, air-displacement pipets are used to quantitatively dispense sample and reagent aliquots for reaction, routine analyses, and specialized tests. Since concentrations of biological and chemical components in the prepared samples for analyses and assays are volume-dependent, incorrectly performed pipetting steps will directly impact the transferred volumes, and hence, the test results.
Working with Artel, Shire developed an analyst qualification program using the same technology it uses to verify and calibrate its robotic liquid handlers. The implementation of the comprehensive program not only reduced assay failures but also helped the company transfer assays from bench to automation by using the same measurement standard for each phase of the process.
The pipette is a reliable precision instrument that has been used and trusted for many years. However, as with many forms of instrumentation, a pipette will perform only as well as the operator’s technique allows. Differences in technique – some more than others – can alter delivery volumes. With increasing demand for accuracy, quality and […]
Any work environment including the laboratory, can be a source of ergonomic stress. Laboratorians that use pipettes frequently may find not only their health, but their proficiency at risk. Two factors in pipetting which can cause the user physical stress are the design of the pipette and the manner in which it is used. An ergonomically […]
Language influences thinking and careful use of words when evaluating data allows us to understand how best to improve laboratory accuracy. Accuracy is a particularly important concept because it is foundational to the quality of laboratory measurements. Accuracy invokes an image of something that is correct, reliable and trustworthy. As a conceptual goal, accuracy is universally […]
The performance of mechanical action pipettes must be tested periodically to ensure accurate liquid delivery. The results of such testing then may be compared with pre-established tolerances, and out-of-tolerance conditions corrected. Tolerances that are too strict can cause a large number of so-called “false failures,” where a pipette in good working order produces test results that are […]