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Lab Report 1: How Many Data Points

By Artel

Human beings are creatures of habit We often seek stability and continuity and are wary of change. However, inspection, evaluation, and subsequent change are often necessary for growth and improvement, both inside and outside the laboratory. A worthy project for your laboratory to undertake this year may be to evaluate your pipette checking routine. One […]

Lab Report

Five Good Reasons: the argument for pipetting technique training

By Carle, Bjoern

Liquid handling quality assurance is a critical component of an effective laboratory quality program. While quality assurance efforts in liquid handling typically place much emphasis on pipette calibration, repair, and maintenance, ensuring pipette operator competence is a crucial, albeit often neglected, area. Yet, improper pipetting technique can undermine the quality of a laboratory’s liquid handling processes and potentially compromise the integrity of results as much as malfunctioning […]

Publication

Optimize Volume Transfer Methods to Avoid Reagent Concentration Error

By Albert, K.

Laboratories utilizing liquid handling devices appreciate the efficiency benefits that these important instruments provide. Fast and repeatable volume transfers, however, do not always contribute to accurate assay results. Assays are dependent on reagent concentrations and reagent concentrations are volume- dependent.

Poster

Accuracy Matters When Quantitative, Manually-pipetted Assays Graduate to Automation

By Albert, K., Knapp, L.

A Story in Diagnosing and Troubleshooting Abstract It is often the case that assays are initially performed on the benchtop  using handheld  pipettes  before they graduate, or transfer,  to an automated liquid handler.  Automating a manual method may take time and patience,  but automation will help lower costs, increase  throughput, and potentially avoid errors associated […]

Poster

Accuracy of Serial Dilutions Tested Over a Large Dilution Range

By Knaide, T., Parshley, R., Bradshaw, J., Curtis, R.

The process of serially diluting solutions in microplates is common for many assays including preparation of dose response curves for IC50, drug efficacy testing, etc. There are many critical steps to this type of assay that are carefully controlled, such as contaminant quantitation and prospective drug concentration.

Poster

Rapidly Quantifying Dilutions in Microtiter Plates

By Knaide, T., Bradshaw, J., Rogers, A., Albert, K.

Dilution-based volume transfer steps are commonly performed for many applications, such as dose response and detection limit testing. Because the exact concentrations of target samples in solution are typically not directly measured but assumed from the dilution ratio used, it is important to accurately measure each dilution step so that the ultimate concentrations of those samples are known.

Poster

Understanding Method Transfer Pitfalls

By Knapp, L., Albert, K.

Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation Introduction Assays are usually performed on the benchtop using handheld pipettes before they graduate, or transfer, to an automated liquid handler. Automating a manual method may take time and patience, but automation helps lower costs, increase throughput, and potentially avoids errors associated with a manual method. During the transfer, however, the […]

Presentation

Determining Dilution Accuracy in Microtiter Plate Assays

By Bradshaw, J.T.

Many critical assays are based upon conducting accurate dilutions of key reagents. Accurately knowing sample concentration is critical for properly interpreting experimental results, which can only be obtained if the experimental dilution ratio is known and controlled.

Presentation
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