Search Resources

Topics
Media Types
Order by:     Title     Ascending / Descending | Type     Ascending / Descending

Accuracy of Serial Dilutions Tested Over a Large Dilution Range

By Knaide, T., Parshley, R., Bradshaw, J., Curtis, R.

The process of serially diluting solutions in microplates is common for many assays including preparation of dose response curves for IC50, drug efficacy testing, etc. There are many critical steps to this type of assay that are carefully controlled, such as contaminant quantitation and prospective drug concentration.

Poster

Rapidly Quantifying Dilutions in Microtiter Plates

By Knaide, T., Bradshaw, J., Rogers, A., Albert, K.

Dilution-based volume transfer steps are commonly performed for many applications, such as dose response and detection limit testing. Because the exact concentrations of target samples in solution are typically not directly measured but assumed from the dilution ratio used, it is important to accurately measure each dilution step so that the ultimate concentrations of those samples are known.

Poster

Understanding Method Transfer Pitfalls

By Knapp, L., Albert, K.

Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation Introduction Assays are usually performed on the benchtop using handheld pipettes before they graduate, or transfer, to an automated liquid handler. Automating a manual method may take time and patience, but automation helps lower costs, increase throughput, and potentially avoids errors associated with a manual method. During the transfer, however, the […]

Presentation

Determining Dilution Accuracy in Microtiter Plate Assays

By Bradshaw, J.T.

Many critical assays are based upon conducting accurate dilutions of key reagents. Accurately knowing sample concentration is critical for properly interpreting experimental results, which can only be obtained if the experimental dilution ratio is known and controlled.

Presentation

The Effects of Pipette Tip Production & Sterilization Methods on the Retention of Liquids

By Spaulding, B.W., Bradshaw, J.T., Albert, K., Campbell, D., Rodrigues, G.

Plastic pipette tips are ubiquitous pieces of labware commonly used in life science, pharmaceutical, chemistry and many other types of laboratories. Because of their use in such diverse fields, certain qualities have been implemented to meet specific needs. Some of these qualities include sterilization of the tips for biological work, the addition of filters inside the tip to reduce aerosol contamination of the pipette cavity, and reducing the volume of drops retained by the tip’s surface.

Poster

Measuring Residual Volumes Remaining in a Plate After Sample Aspiration

By Khovananth, K., Neilsen, J., Albert, K.

The purpose of this application note is to demonstrate an alternative use for the MVS. As opposed to measuring the accuracy and precision for a volume transfer of sample into a microtiter plate, the MVS is instead employed to measure the volume of sample left behind after attempting to aspirate off the entire sample from each well of a microplate, i.e., residual volume of sample.

Application Note

Errors in Liquid Delivery

By Carle, B., Rumery, D., Davis, A., Sawyer, G., Albert, K., Bradshaw, J.

Many common laboratory procedures require quantitative pipettes and mechanical action air-displacement micropipettes are frequently used for this purpose. The design of these pipettes, however, makes their performance susceptible to sample temperature, as well as to environmental relative humidity and barometric pressure.

Poster
  1. Pages:
  3. 1
  4. 2
  5. 3