Volume Verification of Hamilton MICROLAB® STAR and NIMBUS Automated Liquid Handlers
By Stewart, D., Luedke, R., Godowsky, B. | Application Note
A precise and accurate means for conducting volume verification of automated liquid handlers is an important requirement in life science laboratories, which need to determine if pipetting performance is within expected parameters and meets the manufacturers’ published specifications. While gravimetric-based methodologies have historically been the “gold standard” for volume verification, they can represent a difficult and tedious process for end users, are highly sensitive to environmental conditions, and have proved deficient when considering low (sub microliter) volumes. Perhaps most important, gravimetry does not yield accuracy and precision data on a per-channel basis for multi-channel head dispensing. Many labs seeking higher confidence in assay performance therefore demand enhanced volume verification procedures that are less affected by temperature or humidity, more streamlined and easier to perform.