Verifying Mosquito Nanoliter Liquid Dispensing
By Schenker, B., Albert, K., Knaide, T. | Poster
Assay-ready plates are becoming key to maximizing throughput and assay robustness for many HTS departments. These plates contain test compounds prepared at specific concentrations from library stocks ahead of time. DMSO is routinely used for compound dissolution, but its presence at concentrations above 1% can markedly affect assay performance. Thus, accurate volume transfer of nanoliter quantities of DMSO-based stock solution are required to keep concentrations low when preparing assay-ready plates. Sub-microliter liquid handling is technically challenging and requires accurate verification to within a few nanoliters, which is not easily achieved using traditional approaches. mosquito uses positive displacement, disposable tips to aspirate and dispense volumes down to 50 nL. Artel MVS was used to verify mosquito’s precision and accuracy across a range of aqueous and DMSO target volumes for both wet and dry dispensing.