Glycerol Solutions for MVS® Volume Verifications

By Keith J. Albert, Ph.D. | Application Note

Introduction

Glycerol plays an important role in many industries and because of its physical and chemical characteristics, it is used extensively in life science industries. Pharmaceutical and other life science laboratories use glycerol for innumerable sample preparations and assays. Glycerol is often combined and/or used as an additive in aqueous-based solutions and these solutions are used for many types of experiments. For purposes of this application note, the term glycerol solution is employed to refer to an aqueous sample solution with less than 20% glycerol by volume. Aliquots and target volumes of glycerol solutions are usually transferred to the sample holder or assay test plate using a pipettor, such as a multichannel handheld pipette or an automated liquid handler. In many cases, accurate transfer of the target volumes is vital to the integrity of the assay. More importantly, many laboratories do not have an easy and rapid way to verify the accuracy and precision of the pipetting equipment, especially for complex reagents such as glycerol solutions. Described herein is a method to employ the Artel Multichannel Verification System (MVS®) to measure the volume of glycerol solution transferred from a pipettor under test into a microtiter plate. Much of the information in this document has been explained in detail in reference 1.

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