Evaluation of Forward & Reverse Mode Liquid Handling Techniques

By Knaide, T. | Application Note

Introduction

Traditionally, precision of volume dispensing has received more focus than accuracy, making the latter overlooked. This study highlights the importance of understanding both accuracy (how close the transferred volume is to the target volume) and precision (how repeatable the volume transfer is) when evaluating the quality of instrument performance. Quantifying both parameters allows liquid handling techniques to be directly compared. Until now, however, a fast, easy way to measure both accuracy and precision of delivered volumes has been absent. The MVS streamlines the assessment of accuracy and precision into one measurement and facilitates liquid handler optimization through both manual and electronic method adjustments during assay development and on-the-fly assay validation.

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12A5569