Do Plate Readers Agree?
By Knaide, T., Bradshaw, J., Curtis, R., Ritter, G., Parshley, R. | Poster
Understanding Performance Differences between Different Plate Reader Makes and Models
Multiple plate readers are often used throughout a facility to measure the response in microplates for different assay types. However, QC of plate readers is often considered sufficient when PM service is performed or measurement of reader-specific calibration plates is completed for each individual reader. The performance of multiple plate readers, regardless of make or model, is often not directly compared even if those readers are performing the same task(s) for the same assay(s). Unknown performance differences between plate readers introduces an additional source of error to assays at perhaps the most critical step.
In this poster presentation, a method of measuring plate reader performance using Artel MVS dye solutions is described using six different plate reader types from different manufacturers. Using this method, groups of plate readers may be assessed and standardized, thereby minimizing the uncertainty contributed to assay results from plate reader measurements.
It should be noted that this procedure may be employed using different dyes at other wavelengths. In the example described, MVS Sample Solutions were used because they represent the wavelength of interest and are reliably manufactured. When repeating this procedure in an effort to standardize plate readers used for specific assays, appropriate dye solutions should be employed.