From the Reproducibility Initiative to F1000 Research’s Preclinical Reproducibility and Robustness channel, much attention in the life science community has been focused on study reproducibility. These highly publicized efforts cover actions that only a subset of members of the community can take, such as validating important studies and publishing negative results. But data quality is […]
Thanks to equipment advances made to meet the demands of high throughput screening, the use of automation in modern drug discovery and research labs has become commonplace. However, as sample density increases in screening novel drug candidates, humans become more removed from the screening process and proper sample care can occasionally be overlooked. One of […]
This article was originally published in Lab Manager. Problem: In virtually all research processes in which analytes and reagents are mixed in microplates, precise information on the starting volume in each plate well is critical to the accuracy and success of the process, as well as the results derived from it. While effective tools for […]
Research shows that up to 30 percent of pipettes and other liquid delivery devices currently in service are not performing within expected tolerances at any given moment. The risk of non-performing liquid delivery devices is compounded by the ever smaller volumes typically handled in today’s laboratories. This means that volumes that are inaccurate by just a few microliters can have significant effects on results.
To reduce liquid handling error, laboratories must implement regular calibration programs and verification checks for volume transfer accuracy and precision and to quickly identify those systems that are failing. The evaluation method should be standardized, fast, easy to implement, and minimize instrument downtime and required resources.
Every day, air-displacement pipets are used to quantitatively dispense sample and reagent aliquots for reaction, routine analyses, and specialized tests. Since concentrations of biological and chemical components in the prepared samples for analyses and assays are volume-dependent, incorrectly performed pipetting steps will directly impact the transferred volumes, and hence, the test results.
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays by making small but deliberate changes to assay volume delivery. Specifically, protein binding (streptavidin) and […]
Dimethyl sulfoxide (DMSO) has become one of the most important solvents used for high-throughput screening (HTS) and compound management applications due to its ability to readily dissolve a wide range of compounds. It is also the solvent of choice for the new breed of acoustic droplet ejection (ADE) liquid handling instruments, developed by companies such […]
Liquid handling quality assurance is a critical component of an effective laboratory quality program. While quality assurance efforts in liquid handling typically place much emphasis on pipette calibration, repair, and maintenance, ensuring pipette operator competence is a crucial, albeit often neglected, area. Yet, improper pipetting technique can undermine the quality of a laboratory’s liquid handling processes and potentially compromise the integrity of results as much as malfunctioning […]