Is your IC50 really an IC50? Do you know?
Artel simplifies the verification of low-volume DMSO dispenses and improves the validity of HTS assays
Research by North Carolina State University’s Dr. Nathaniel Hentz has shown that the volumes dispensed during drug screening programs can have a significant effect on the results obtained by assay screens. Any error made during these deliveries affects the concentration of the drug candidate and can lead to misleading data about compound activity being generated.
Because DMSO has very different physical properties than water it behaves differently when handled by air-displacement pipettors or acoustic droplet ejectors, creating the need for a solvent-specific volume verification method such as that provided by the new DMSO Range E Solution for the MVS.
DMSO-based Sample Solutions Will Help You:
- Ensure concentrations of compounds in assay plates by measuring nL volumes of 100% DMSO
- Measure performance of acoustic dispensing systems and other low-volume liquid dispensers
- Standardize nL volume delivery across liquid handler types with DMSO- and aqueous-based solutions
Our laboratory has used Artel’s MVS for the last five years to validate volume transfers for our automated liquid handling devices. While the MVS has proved valuable as an easy to use aqueous liquid volume validation, most of our liquid transfers involve moving our compound library which is solvated in DMSO. We are eagerly looking forward to Artel’s new DMSO-based Sample Solutions to make it even easier to validate the liquid transfers of DMSO solutions on our systems.
Jason Haelewyn, Celgene